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The transfection protocol was consistent with previous reports ( 2.13 Western blot Proteins were extracted from colon tissue and cell samples and stored at 20C

This may include staying hydrated, maintaining a balanced diet, and monitoring for any unusual reactions

These can range from acute infectious diseases with short durations of days to weeks (depending on severity and location of the infection) to chronic diseases (eg, kidney or heart disease) that cannot be cured but can be managed and last the duration of the pets lifetime

Among them, bacterial LOXs remain underexplored compared to their eukaryotic counterparts, representing an untapped resource with unique structural and functional traits